Journal: eLife

Article Title: A neurotrophin functioning with a Toll regulates structural plasticity in a dopaminergic circuit

doi: 10.7554/eLife.102222

Figure Lengend Snippet:

Article Snippet: Standard qPCR was performed with SensiFast syb Green Mix (Bioline #B2092020) in ABI qPCR plate (GeneFlow #P3-0292) and machine.

Techniques: CRISPR, Reverse Transcription, Plasmid Preparation, Software

Journal: Cells

Article Title: A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts

doi: 10.3390/cells10102705

Figure Lengend Snippet: Differential expression of lncRNAs between ADAM15-expressing and non-expressing synovial fibroblasts (SF) under mechanical strain. ( A – D ) SF ( n = 4), either expressing ADAM15 or downregulated with a specific siRNA, were strained for 3 h using the Flexcell System (elongation 15%, frequency 1 Hertz). Reverse-transcribed cDNAs were then amplified in Arraystar lncRNA PCR-plates, and the fold change of up-/down-regulation of gene expression was calculated with the 2 −∆∆Ct method. ( A,C ) Top 20 lncRNAs differentially up- and downregulated in ADAM15-expressing versus non-expressing SF. ( B,D ) Venn diagram of all differentially expressed lncRNAs with a 2-fold change up/downregulation, identifying HOTAIR and H19-2 as differentially downregulated lncRNAs in all 4 SF (underlined in C ; intersection, boxed in D ). Intersections among 3 donors are shown in grey.

Article Snippet: SF from 4 different donors, pretreated with either specific siRNA for ADAM15 or non-silencing control siRNA were strained for 3 h. Subsequently, transcribed RNA was amplified using Arraystar lncRNA qPCR plates coated with primers for 372 disease-relevant lncRNAs, and the overall top 20 up-/downregulated lncRNAs from all 4 donors were determined ( A,C).

Techniques: Quantitative Proteomics, Expressing, Reverse Transcription, Amplification, Gene Expression

Journal: Cell Host & Microbe

Article Title: Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike protein

doi: 10.1016/j.chom.2022.07.016

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Article Snippet: 96-well qPCR plate , Corning , AXYPCR96LC480WNF.

Techniques: Virus, Recombinant, Expressing, Transfection, Staining, Plasmid Preparation, DNA Purification, Cloning, Software, Binding Assay, Sterility, Magnetic Beads, Cell Culture, Colony Assay